解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Characterization of protein damage during photosensitization of chlorin e6-treated cells was performed using the green fluorescent protein (GFP). The GFP-chromophore damage caused by singlet oxygen was studied in COS 7 kidney cells and E. coli bacteria following light irradiation. Electron spin resonance (ESR) reveale...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2000.153
更新日期:2000-12-01 00:00:00
abstract::The GAGA box of the rat serine protease inhibitor 2 (spi 2) genes not only acts as a basal promoter element, but also mediates transcriptional activation by growth hormone and interleukin-6. The GAGA box is separated from the TATA box by only 12 bp, and this close association is required for efficient transcription. H...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2000.127
更新日期:2000-11-01 00:00:00
abstract::N.BstNBI is a unique restriction endonuclease isolated from Bacillus stearothermophilus. We have characterized the recognition sequence and the cleavage site of N.BstNBI. Mapping of cleavage sites of N.BstNBI showed that it recognizes an asymmetric sequence, 5' GAGTC 3', and cleaves only on the top strand 4 base pairs...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2000.137
更新日期:2000-11-01 00:00:00
abstract::Plastids originated from an endosymbiotic event between an early eukaryotic host cell and an ancestor of today's cyanobacteria. During the events by which the engulfed endosymbiont was transformed into a permanent organelle, many genes were transferred from the plastidal genome to the nucleus of the host cell. Protein...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2000.110
更新日期:2000-09-01 00:00:00
abstract::Thyroid hormones stimulate gap junctional communication in rat liver WB-F344 epithelial cells, elevating connexin43 mRNA and protein levels. In the present work we analysed connexin43 expression in liver and heart samples from thyroid hormone-treated Wistar rats. Connexin43 mRNA was elevated 2.1-fold in rat liver samp...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2000.120
更新日期:2000-09-01 00:00:00
abstract::Plants lack specialised organs and circulatory systems, and oxygen can fall to low concentrations in metabolically active, dense or bulky tissues. In animals that tolerate hypoxia or anoxia, low oxygen triggers an adaptive inhibition of respiration and metabolic activity. Growing potato tubers were used to investigate...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2000.093
更新日期:2000-08-01 00:00:00
abstract::The transcription factor GLI1 is one of three vertebrate members of the GLI family, which is characterized by a highly conserved DNA-binding domain of five zinc fingers. We have analyzed whether human GLI1 is a target of PKA regulation. It was found that PKA inhibits GLI1 transcriptional activity. However, no evidence...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2000.070
更新日期:2000-07-01 00:00:00
abstract::In mammals, betaine of the mitochondrial matrix is used in the cytosol by betaine-homocysteine S-methyltransferase for methionine synthesis. The resulting dimethylglycine is shuttled back into the mitochondrial matrix for further degradation. Nanospray tandem mass spectrometry and N-terminal amino acid sequencing of m...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2000.080
更新日期:2000-07-01 00:00:00
abstract::Among the Ras family, Ran is a unique small G protein. It does not have a lipid modification motif at the C-terminus to bind to the membrane, which is often observed within the Ras family. Ran may therefore interact with a wide range of proteins in various intracellular locations. This means that Ran could play many d...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2000.052
更新日期:2000-05-01 00:00:00
abstract::The enzymatic properties of glycosylphosphatidylinositol-specific phospholipase D (EC 3.1.4.50) were characterized using a 6,000-fold purified enzyme. This was obtained in 100 microg amounts from human serum with a recovery of 35%. Pure alkaline phosphatase containing one anchor moiety per molecule was used as substra...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2000.062
更新日期:2000-05-01 00:00:00
abstract::The relationship between the structure and activity of meta- and para-hydroxylated monophenols was studied during their tyrosinase-catalysed hydroxylation and the rate-limiting steps of the reaction mechanism were identified. The para-hydroxylated substrates permit us to study the effect of a substituent (R) in the ca...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2000.041
更新日期:2000-04-01 00:00:00
abstract::The stress-activated protein kinase p38/SAPK2 is known to regulate the activity of transcription factors and to control expression of several genes at the transcriptional or post-transcriptional level. In order to identify genes whose expression is under the control of p38/SAPK2 activity, we have compared the mRNA lev...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2000.026
更新日期:2000-03-01 00:00:00
abstract::Latent episomal genomes of Epstein-Barr virus, a human gammaherpesvirus, represent a suitable model system for studying replication and methylation of chromosomal DNA in mammals. We analyzed the methylation patterns of CpG dinucleotides in the latent origin of DNA replication of Epstein-Barr virus using automated fluo...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2000.014
更新日期:2000-02-01 00:00:00
abstract::Functional ribonuclease A was presented on the surface of the filamentous phage M13 by fusion to the minor coat protein. RNase activity of the fusion protein was shown by a zymogram assay. In addition, we established a modified method for preparing RNase-displaying phages without contaminating host RNases. ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2000.024
更新日期:2000-02-01 00:00:00
abstract::Isolated human granulocyte plasma membranes contain progelatinase B. The binding of progelatinase B to the membrane, however, is relatively weak, and a considerable part of progelatinase B can be removed by simply washing the membrane with buffer. This detachment does not depend on the ionic strength of the buffer, in...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2000.007
更新日期:2000-01-01 00:00:00
abstract::The albumin gene family arose in a series of duplication events which gave rise to symmetry in its structure. The four genes are tandemly linked on human chromosome 4q in the order: 5'ALB-5'AFP-5'ALF-5'DBP-centromere, and their introns display a symmetrical and repetitive pattern that is shared by members of the gene ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.1999.183
更新日期:1999-12-01 00:00:00
abstract::A deletion mutant (V314) of the calcineurin A subunit was constructed using site-directed mutagenesis. Its phosphatase activity and function were then characterized. The V314 deletion significantly altered the phosphatase activity, which was more than ten times higher than that of wild-type calcineurin, the calcineuri...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.1999.163
更新日期:1999-11-01 00:00:00
abstract::Dog pancreas microsomes represent the key components of the established model system for the analysis of protein transport into the mammalian endoplasmic reticulum. More recently, these microsomes were also employed in cell-free systems which address questions related to protein folding and protein degradation in the ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.1999.149
更新日期:1999-10-01 00:00:00
abstract::The T-knot scaffold, a disulphide-reinforced structural motif shared by several proteins with very different biological functions, has been defined as 'a stretch of the protein chain which comprises two strands of a beta-sheet and three loops, knotted by two disulphides into the shape of the letter T'. In this communi...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.1999.159
更新日期:1999-10-01 00:00:00
abstract::There is increasing evidence that apoptosis and necrosis represent only two of several possible ways for cells to die. These two types of demise can occur simultaneously in tissues or cell cultures exposed to the same stimulus, and often local metabolic conditions and the intensity of the same initial insult decide th...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.1999.129
更新日期:1999-09-01 00:00:00
abstract::Protein phosphatase 2A (PP2A) constitutes one of the major families of protein serine/threonine phosphatases found in all eukaryotic cells. PP2A holoenzymes are composed of a catalytic subunit complexed with a structural regulatory subunit of 65 kDa. These core subunits associate with regulatory subunits of various si...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.1999.139
更新日期:1999-09-01 00:00:00
abstract::Type I allergy, a hypersensitivity disease affecting almost 20% of the population worldwide, is based on the IgE recognition of otherwise harmless antigens (i.e., allergens). Allergen-induced crosslink of effector cell-bound IgE antibodies leads to the release of biological mediators and thus to immediate disease symp...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.1999.101
更新日期:1999-07-01 00:00:00
abstract::In pancreatic acinar cells stimulation of different intracellular pathways leads to different patterns of Ca2+ signaling. Bombesin induces activation of both phosphatidylinositol 4,5-bisphosphate (PIP2)-specific phospholipase C (PLC) and phospholipase D (PLD). The latter leads to generation of diacylglycerol (DAG) in ...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.1999.111
更新日期:1999-07-01 00:00:00
abstract::Hyperpolarization-activated cation currents, termed I(h), are observed in a variety of neurons. Four members of a gene family encoding hyperpolarization-activated cyclic-nucleotide-gated cation channels (HCN1-4) have been cloned. The regional expression and cellular localization of the four HCN channel types in mouse ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.1999.121
更新日期:1999-07-01 00:00:00
abstract::The cellular adhesion molecule E-selectin is expressed on activated endothelial cells, and is involved in the process of adherence of blood cells to vessel endothelium in inflammatory events such as atherosclerosis. In a recent study we found a Ser128Arg mutation in the EGF domain as well as a Leu554Phe mutation in th...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.1999.082
更新日期:1999-06-01 00:00:00
abstract::We studied the mode of regulation of the activity of mature cathepsin B (CB) by L-cysteine and some related thiols. The activity of CB with Z-Arg-Arg-NHMec as substrate was gradually inhibited over a range of increasing concentration of Cys, Cys methyl ester (CysOMe), Cys ethyl ester (CysOEt), N-acetyl-Cys (N-AcCys) a...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.1999.069
更新日期:1999-05-01 00:00:00
abstract::Glycoconjugates have a whole spectrum of biological roles, from those that appear trivial to those that are crucial. Results accumulated in the past years indicate they might also play an important role in the response to stress, a complex physiological response of the human organism to various threats. We have recent...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.1999.058
更新日期:1999-04-01 00:00:00
abstract::Vaccination with either exogenous hepatitis B surface antigen (HBsAg) lipoprotein particles without adjuvants, or plasmid DNA encoding secreted small HBsAg stimulate long-lasting, potent antibody responses in H-2d (BALB/c) and C57Bl/6 (H-2b) mice. Vaccination with exogenous HBsAg primes MHC-I restricted cytotoxic T ly...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.1999.039
更新日期:1999-03-01 00:00:00
abstract::DNA-based immunization has been shown to induce protective immunity against several microbial pathogens including HIV-1. Several routes of DNA vaccination have been exploited. However, the properties of the immune responses seem to differ with the different routes used for DNA delivery, ultimately affecting the outcom...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.1999.049
更新日期:1999-03-01 00:00:00
abstract::The signal recognition particle (SRP), a ubiquitous cytoplasmic ribonucleoprotein particle, plays an essential role in promoting co-translational translocation of proteins into the endoplasmic reticulum. Here, we summarise recent progress made in the understanding of two essential SRP functions: the signal recognition...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.1999.021
更新日期:1999-02-01 00:00:00
abstract::We have cloned from a chicken intestinal cDNA library Cmdr1, the first avian P-glycoprotein. Cmdr1 is 67% and 69% identical to proteins encoded by the human MDR1 and MDR2 genes, respectively. Functional expression of Cmdr1 in both mouse NIH 3T3 and yeast cells demonstrated that Cmdr1 represents the avian ortholog of h...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.1999.031
更新日期:1999-02-01 00:00:00
abstract::We have investigated the methylation status of the repeat unit of the human ribosomal RNA genes in lung cancer. Using a Southern blot analysis approach we have determined that the non-transcribed region of these genes was generally heavily methylated, while the transcribed region was not methylated in either tumor or ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.1999.010
更新日期:1999-01-01 00:00:00
abstract::The three-dimensional structure of pterin-4a-carbinolamine dehydratase (PCD) from Pseudomonas aeruginosa has been solved. Based on this we have investigated the roles of putative active center residues through functional replacement by site-directed mutagenesis. Three histidines, His73, His74 and His91, appear to be i...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/bchm.1998.379.12.1427
更新日期:1998-12-01 00:00:00
abstract::The bifunctional protein DCoH (dimerization cofactor of HNF-1) is a transcriptional coactivator of homeodomain proteins and a cytoplasmic enzyme. Here we report on the isolation, nucleotide sequence and genomic structure of the chicken and rat DCoH genes. They have an unusual first exon coding for a single amino acid....
journal_title:Biological chemistry
pub_type: 杂志文章
doi:
更新日期:1998-11-01 00:00:00
abstract::We present a rapid and simple method for the sexing of human and other primate DNA. Homologous introns from the X- and Y-linked zinc finger protein genes (ZFX and ZFY) are PCR amplified with one primer pair. An Alu element insertion in the ZFX intron results in a size difference in the amplicons which is resolved by a...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:
更新日期:1998-10-01 00:00:00
abstract::This review deals with current concepts on the regulation and function of phospholipid asymmetry in biological membranes. This ubiquitous phenomenon is characterized by a distinctly different lipid composition between the inner and outer leaflet of the membrane bilayer. Transbilayer asymmetry is controlled by differen...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:
更新日期:1998-08-01 00:00:00
abstract::Mammalian apolipoproteinB (apoB) RNA editing is a site-specific deamination reaction that mediates the C to U conversion responsible for apoB48 production in the mammalian small intestine. This process is not detected in chicken apoB RNA. Mammalian apoB RNA editing is mediated by a multicomponent enzyme complex that i...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/bchm.1998.379.8-9.1075
更新日期:1998-08-01 00:00:00
abstract::Animal cells release traces of material onto glass or silicon surfaces during adhesion and migration. This little studied phenomenon is a widespread and normal concomitant of cell migration. The paper introduces the study of such material. The traces can be visualised by different microscopic techniques (e.g. TIRF, IR...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/bchm.1998.379.8-9.1161
更新日期:1998-08-01 00:00:00
abstract::The central process for the transfer of the genetic information from the nucleic acid world into the structure of proteins is the ribosomal elongation cycle, where the sequence of codons is translated into the sequence of amino acids. The nascent polypeptide chain is elongated by one amino acid during the reactions of...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/bchm.1998.379.7.753
更新日期:1998-07-01 00:00:00
abstract::The influence of osmolarity and compatible organic osmolytes on the phosphorylation of the MAP-kinases Erk-1 and Erk-2 and on the expression of taurine transporter (TAUT) and lipopolysaccharide (LPS)-induced nitric oxide synthetase (iNOS) was studied in RAW 264.7 mouse macrophages. Hypoosmolarity (205 mosmol/l) but no...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/bchm.1998.379.7.867
更新日期:1998-07-01 00:00:00